Molecular mechanisms of mesenchymal stem cell differentiation towards osteoblasts

role of microrna in mesenchymal stem cells differentiation into osteoblasts

results treatments through mirnas can prove to be beneficial due to its small size, easy synthesis, targeted and quick delivery. conclusions further work is needed to be done to make mirna treatments available to large number of people suffering from various bone diseases. context mirnas can regulate the expression of genes by interfering in their mrnas, acting as key regulators of diverse cell...

Gene amplification in mesenchymal stem cells and during differentiation towards adipocytes or osteoblasts

Gene amplifications are an attribute of tumor cells and have for long time been overlooked in normal cells. A growing number of investigations describe gene amplifications in normal mammalian cells during development and differentiation. Possibly, tumor cells have rescued the gene amplification mechanism as a physiological attribute of stem cells. Here, we investigated human mesenchymal stem ce...

Cellular and Molecular Mechanisms of Mesenchymal Stem Cell Actions

Glycans modify mesenchymal stem cell differentiation to impact on the function of resulting osteoblasts.

Glycans are inherently heterogeneous, yet glycosylation is essential in eukaryotes, and glycans show characteristic cell type-dependent distributions. By using an immortalized human mesenchymal stromal cell (MSC) line model, we show that both N- and O-glycan processing in the Golgi functionally modulates early steps of osteogenic differentiation. We found that inhibiting O-glycan processing in ...

Para-Nonylphenol Impairs Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells by Influencing the Osteoblasts Mineralization

Objective(s)Para-Nonylphenol (p-NP) is used in many industries and our previous study showed that p-NP causes a reduction in rats bone marrow mesenchymal stem cells (MSCs) viability. The aim of this study was to investigate the effect of p-NP on osteogenic differentiation of MSCs.Materials and MethodsMSCs were isolated and expanded to 3rd passage, then cultured in DMEM supplemented with osteoge...